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Korean Journal of Clinical Oncology > Article
Korean J Clin Oncol. 2011;7(2): 96-107.         doi: https://doi.org/10.14216/kjco.11025
Microsatellite Alterations of Major Tumor Suppressor Genes in Hepatocellular Carcinoma
Jang Hyuk Seo1, Doo Jin Kim1, Seong Jin Cho2, Ji Hyun Kwon2, Eun Sook Nam2, Hyung Sik Shin2, Joo Seop Kim1
1Department of Surgery, Hallym University College of Medicine, Seoul
2Department of Pathology, Hallym University College of Medicine, Seoul
간세포암에서 주요 종양억제 유전자에서의 현미부수체 변이
서장혁1, 김두진1, 조성진2, 권지현2, 남은숙2, 신형식2, 김주섭1
1한림대학교 의과대학 외과학교실
2한림대학교 의과대학 병리학교실
Corresponding Author: Joo Seop Kim ,Tel: +82-2-2224-2450, Fax: +82-2-2224-2570, Email: jskim@hallym.or.kr
Received: September 23, 2011;  Accepted: October 20, 2011.
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Purpose : Hepatocellular carcinoma (HCC) shows various molecular and genetic alterations in its development and progression. The major tumor suppressor genes (TSGs) such as p16, PTEN, E-cadherin and p53 on chromosomes 9p21, 10q23, 16q22, 17p13 may play important roles in various regulation pathways as well as tumor suppression. Microsatellite alteration such as loss of heterozygosity (LOH) has been reported is a predominant mechanism of inactivation of TSGs. Also, LOH is known to be related with allelic loss of various TSGs. This study evaluated LOH of 4 TSGs in HCCs and correlated these results with the clinicopathological factors. Methods : LOH analysis was carried out using a polymerase chain reaction with 16 polymorphic microsatellite markers of 4 chromosomes having TSGs in 50 surgically resected tumors and their non-tumorous counterparts. Results : There was no detectable LOH in normal tissue. LOH was detected in 82% of 50 cases of HCCs. LOH of 9p21, 10q23, 16q22, 17p13 was detected in 36%, 54%, 38% and 66%, respectively. Advanced HCCs showed significant LOH results of 9p21, 10q23 & 17p13. According to tumor grade, those of 10q23, 16q22, 17p13 correlated with higher grade III. Vascular invasion & lymphatic emboli showed correlation with those of 9p21, 10q23, 17p13. Cancer recurrence & metastasis significantly correlated with 9p21 LOH. Conclusion : These results suggest that LOH of various chromosomes may be predominantly involved in hepatocarcinogenesis and tumor invasion. Also, those LOH may be useful clinical indicator in determining the poorer prognosis among patients with HCC.
Keywords: Carcinoma | Hepatocellular | Chromosomes | Human | 9 | 10 | 16
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